Περιεχόμενα
Introduction. The beginnings of human in vitro fertilization. Robert Edwards: The path to IVF. Establishing and Maintaining an IVF Laboratory. Setting up an ART laboratory. Quality control: Maintaining stability in the laboratory. The ART laboratory: Current standards. Gamete Collection, Preparation and Selection. Evaluation
of sperm. Sperm preparation techniques. Sperm chromatin assessment.
Oocyte retrieval and selection. Preparation and evaluation of oocytes
for ICSI. Hyaluronic acid binding-mediated sperm selection for ICSI. Use
of in vitro maturation in a clinical setting: Patient populations and
outcomes. Micromanipulation. Micromanipulation. Intracytoplasmic sperm
injection: Technical aspects. Assisted hatching. Human embryo biopsy
procedures. Culture, Selection and Transfer of the Human Embryo. Oocyte
activation. Analysis of fertilization. Culture systems for the human
embryo. Evaluation of embryo quality: Analysis of morphology and
quantification of nutrient utilization and the metabolome. Evaluation of
embryo quality: Time-lapse imaging to assess embryo morphokinetics.
Evaluation of embryo quality: Proteomic strategies. Cryopreservation.
The human oocyte: Controlled rate cooling. The human oocyte:
Vitrification. The human embryo: Slow freezing. The human embryo:
Vitrification. Managing an oocyte bank. Diagnosis of Genetic Disease in Preimplantation Embryos. Severe
male factor: Genetic consequences and recommendations for genetic
testing. Polar body biopsy and its clinical application. Preimplantation
genetic diagnosis for infertility. Genetic analysis of the embryo.
Implantation. The analysis of endometrial receptivity. Future Directions and Clinical Applications. Artificial
gametes: The sperm. Artificial gametes: The oocyte. Rejuvenating
oocytes with young mitochondria. Chemical stimulation of the aged
oocyte. Microfluidics in ART: Towards automation of the IVF laboratory.
Quality Management Systems. Quality management in reproductive medicine.
Patient Investigation and the Use of Drugs. Lifestyle, periconception,
and fertility. Indications for IVF treatment: From diagnosis to
prognosis. Initial investigation of the infertile couple. Fertility gene
mapping. Prognostic testing for ovarian reserve. Drugs used for ovarian
stimulation: Clomiphene citrate, aromatase inhibitors, metformin,
gonadotropins, gonadotropin-releasing hormone analogs, and recombinant
gonadotropins. The role of FSH and LH in ovulation induction: Current
concepts. Stimulation Protocols. Endocrine characteristics of ART
cycles. The use of GnRH agonists. GnRH-antagonists in ovarian
stimulation for IVF. The use of AMH to tailor ovarian stimulation for
IVF. Monitoring ovarian response in IVF cycles. Oocyte collection.
Luteal support in ART. Treatment strategies in assisted reproduction for
the poor responder patient. Repeated implantation failure. Technical
Procedures and Outcomes. Ultrasonography in assisted reproduction. Sperm
recovery techniques: Clinical aspects. Processing and cryopreservation
of testicular sperm. Embryo transfer technique. Cycle regimes for
frozen–thawed embryo transfer. Managing freeze all oocytes. Anesthesia
and in vitro fertilization. Medical considerations of single embryo
transfer. Special Medical Conditions. Endometriosis and ART. PCOS and
assisted reproduction. Management of hydrosalpinx. Fertility
preservation strategies. Viral disease and Assisted Reproductive
Techniques. Complications of Treatment. Severe ovarian hyperstimulation
syndrome. The environment and reproduction. Bleeding, severe pelvic
infection, and ectopic pregnancy. Iatrogenic multiple pregnancies: The
risk of ART. 3D Ultrasound. Non-invasive prenatal testing. Egg Donation
and Surrogate Motherhood. Egg and
