Cryo-Electron Microscopy in Structural Biology. From Structural Insights to Tomography and Drug Discovery

Περιεχόμενα

SECTION I: Cryo-EM in Cell Biology – Structure Determination of Cellular Organelle 1. Atomic-resolution protein structure determination by cryo-EM 2. Sub-ångström cryo-EM Structure of a prion proto-fibrils 3. Cryo-EM structure of native human uromodulin, a zona pellucida module polymer 4. Chromatosome, telomere structure and genome maintenance using cryo-EM 5. Cryo-EM of the dynamin super-family proteins 6. Mito-ribosome structure using cryo-EM SECTION II: Technology Platform & Evolving Technologies 1. Cryo-EM data in the cloud computing 2. The silent revolution of cryo-EM and GPCR structures 3. Development of world-first multi-gas plasma focused ion beam microscope 4. 3-D printed cell culture grid holders for improved cellular specimen preparation in cryo- electron microscopy 5. Local resolution estimates of cryo-EM reconstruction 6. A new cryo-EM system for electron 3D crystallography SECTION III: Cryo-EM in Virology – High-Resolution Structures of Viruses 1. Cryo-Electron Microscopy structure of adeno-associated virus-DJ bound by a heparinoid pentasaccharide 2. Cryo-EM and tomography of vesicular stomatitis viruses 3. High-resolution structures of HIV-1 gag cleavage mutants 4. Cryo-electron microscopy of the T7 bacteriophage portal and fiber-less tail complex 5. SARS-Co-2 spike protein structure determination SECTION IV: Cryo EM in Membrane Biology – Probing the structures of Membrane Proteins 1. Structure and activity of lipid bilayer within a membrane-protein transporter 2. Cryo-EM structure of human Rhodopsin bound to an inhibitory G protein 3. Cryo-EM structure of the acetylcholine receptor that cause congenital myasthenic syndromes 4. Structure of the mitochondrial F-ATPase as determined by electron cryo-microscopy 5. Cryo-EM structure of cardiac amyloid fibrils from an immunoglobulin light chain AL amyloidosis patient SECTION V: Cryo-EM in Molecular Biology – Structural Insights of Nuclear and Gene Machineries 1. Structural information from cryo-EM studies on multicomponent molecular assemblies such as those found in DNA-double-strand-break repair 2. Cryo-EM structure of the DNA-PK holoenzyme 3. Cryo-EM reconstruction at 4.0 Å of human Polymerase III 4. Cryo-electron microscopy structures of UV-DDB bound to nucleosome 5. Cryo-electron microscopy of the chromatin fiber 6. Time-resolved cryo-EM studies in the ribosome dynamics SECTION VI: Cryo-Tomography and Emerging Technologies 1. Cryo-Electron Tomography in membrane protein structural research 2. Cryo-tomography of membrane transporters involved in neurological psychiatric disease 3. Enveloped viruses and coated vesicles – electron cryo-microscopy and tomography 4. Electron cryo-tomography in the study of autophagy or bacterial secretion systems 5. DNA Origami sign posts for identifying proteins on cell membranes by cryo-tomography 6. 3-D deconvolution processing for STEM cryo-tomography SECTION VII: Applications of Cryo-EM/ET in Drug Discovery 1. Cryo-EM to enable research and drug discovery of CD20 in complex with the therapeutic monoclonal antibody rituximab 2. Fragment-based drug discovery using cryo-EM 3. CryoEM approaches to study allosteric regulation of GTP cyclohydrolase I 4. Cryo-EM in drug discovery 5. Structural insights of an advanced vaccine candidate as derived from SARS-CoV-2 spike protein 6. Unleashing the power of Cryo-EM Epilogue