Περιγραφή
In-vitro fertilization (IVF) is a procedure practised worldwide, with patients and practitioners expecting higher live birth rates and excellent standards of quality as methodologies become more established. This practical book covers every stage in the life of an IVF laboratory, from designing and planning the laboratory to training the embryologists and ongoing management and troubleshooting, providing step-by-step protocols for today’s best practice. Chapters follow the same structure, allowing readers to translate complex and specialized procedures into a standard workflow. Several new chapters are included in this second edition, including descriptions of the protocol for piezo-ICSI, safe and effective cryostorage of embryos, and how to manage emergency situations in the IVF laboratory. Written by an international team of experts, this is an exemplary portfolio of techniques, providing a good foundation of working practice for both new and experienced embryologists.
- Chapters are short and follow a standard structure, with key references highlighted for further reading, allowing readers to absorb relevant information quickly
- Mirroring the life cycle of an IVF laboratory, chapters cover all aspects of set up from the first day of planning a clinic and establishing training protocols to quality control and troubleshooting once daily operations and practices are standardized
- Includes several chapters new to this edition expanding on developments in the field such as piezo-ICSI, safe and effective cryostorage and a chapter on managing emergency situations in the IVF laboratory
Περιεχόμενα
The evolution of IVF practice
Section I. Starting a New Laboratory & Training Protocols:
1. Establishing and equipping a new IVF laboratory
2. Basic embryology skills and responsibilities in the IVF laboratory
3. Sperm preparation for IVF: training protocol
4. Oocyte pick-up for IVF: training protocol
5. Embryologist training: embryo transfer
6. ICSI for IVF: training protocol
7. Embryo assisted hatching for IVF: training protocol and method
8. Embryo biospy for IVF: training protocol
9. Vitrification for IVF: training protocol
10. Mouse embryo assay for quality control in the IVF laboratory
11. Sperm survival assay for quality control in the IVF laboratory
Section II. Pre-Procedure Protocols:
12. Quality control in the IVF laboratory: continuous improvement
13. Setup procedures for optimizing performance in the IVF laboratory
Section III. Gametes:
14. Sperm preparation for therapeutic IVF
15. Processing surgically retrieved sperm in the IVF laboratory
16. Cryopreservation of sperm for IVF: semen samples and individual sperm
17. Oocyte collection for IVF
18. In-vitro maturation of immature oocytes for IVM-IVF
19. Oocyte grading by morphological evaluation
20. Vitrification of oocytes for IVF
Section IV. Insemination/ICSI:
21. General insemination for IVF
22. Conventional IVF with short co-incubation
23. Technical aspects of ICSI for ejaculated spermatozoa
24. Set-up and adapted protocol for piezo-ICSI
25. Sperm selection for ICSI by morphology: MSOME/IMSI
26. Sperm selection for ICSI by viability
27. Artificial oocyte activation for IVF
Section V. Fertilization Assessmant:
28. Fertilization assessment in IVF and ICSI
29. Polar body biopsy for IVF
30. Zygote cryopreservation for IVF
Section VI. Embryo Assessment – Morphology & Beyond:
31. Embryo assessment at the pre-compaction stage in the IVF laboratory
32. Embryo assessment in the post-compaction stage in the IVF laboratory
33. Embryo assessment at the blastocyst stage in the IVF laboratory
34. Trophectoderm biopsy for preimplantation genetic testing
35. Embryo culture by Time-lapse: selection and beyond
Section VII. Embryo Cryopreservation:
36. Cryopreservation of pre-compaction embryos for IVF
37. Cryopreservation of post-compaction embryos for IVF
38. Safe and effective cryostorage of embryos for IVF
Section VIII. Embryo Transfer:
39. Routine embryo transfer in the IVF laboratory
Section IX. Quality Management:
40. Verification of specimen identity by double checking with a witness
41. Quality management in the IVF laboratory: quality improvement, document and process control and adverse events
42. Trouble-shooting in the IVF laboratory
43. Disaster preparedness in the IVF Laboratory.
